Alternative Names:

AKT; AKT1 kinase; C-AKT; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha;

Application:

WB IHC ICC/IF

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:100~1:500

	Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt(Ser473) Antibody.
	Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody.
	Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using Phospho-Akt(Ser473) Antibody.
	Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry
	Fibulin-3 has anti-tumorigenic activities in cutaneous squamous cell carcinoma. -Journal of Investigative Dermatology
	Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation. -Oncology Reports

Purification:

Affinity-chromatography

Specificity:

Phospho-Akt(Ser473) Antibody detects endogenous levels of total Phospho-Akt(Ser473)

Immunogen:

A synthesized peptide derived from human Phospho-Akt(Ser473)

Description:

An AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway. Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. First found in a mouse transforming retrovirus.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

CY6569.pdf